Well,
the error says "authentication failed"...which indicates that the signature that was applied in AS2 could not be verified by the recipient.
Usually for the "signing" part, you would typically use a "Private Key" (and then your partenr uses the corresponding public cert to verify the signature.
However, in the screenshots I looks like you apparently use a public SSL-certificate for signing a AS2 message, which seems a little strange to me.
KR
Stefan